| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Powell, Alyssa V (17)
|
CASBSC |
51.93 |
51.40 |
-0.53 |
| 2 |
Teng, Cathy (13)
|
PCSCSC |
52.31 |
51.91 |
-0.40 |
| 3 |
Weber, Delia M (17)
|
GUFCST |
52.40 |
52.49 |
0.09 |
| 4 |
Marvin, Payton C (13)
|
STNTRO |
53.03 |
52.61 |
-0.42 |
| 5 |
Kelley, Bridget T (17)
|
FLSTAR |
52.53 |
52.87 |
0.34 |
| 6 |
Jennings, Emmie T (17)
|
AKKNIK |
50.80 |
52.90 |
2.10 |
| 7 |
Rumpelt, Natalie H (16)
|
ADALBS |
52.56 |
52.92 |
0.36 |
| 8 |
Wu, Cynthia M (17)
|
NJSCAR |
59.90 |
52.93 |
-6.97 |
| 9 |
Federlin, Jade A (16)
|
STNTRO |
53.25 |
52.98 |
-0.27 |
| 10 |
Kerr, Pauline E (16)
|
NTDM |
52.04 |
53.00 |
0.96 |
| 11 |
Orbach-Mandel, Hannah E (17)
|
AZTJCC |
52.52 |
53.17 |
0.65 |
| 12 |
Koo, Caitlyn (15)
|
PCSCSC |
52.23 |
53.27 |
1.04 |
| 13 |
Stone, Sarah K (17)
|
NTDM |
52.50 |
53.30 |
0.80 |
| 14 |
King, Erica S (18)
|
ILPAC |
53.11 |
53.40 |
0.29 |
| 15 |
Quattlebaum, Peyton R (15)
|
GUFCST |
53.32 |
54.27 |
0.95 |